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reverse transcription kit (catalog number:4368813  (Thermo Fisher)


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    Structured Review

    Thermo Fisher reverse transcription kit (catalog number:4368813
    Reverse Transcription Kit (Catalog Number:4368813, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcript+numbers/pm39624268-66-22-27
    Average 90 stars, based on 1 article reviews
    reverse transcription kit (catalog number:4368813 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Inhibition of caspase-1 prolongs survival of mice infected with rabies virus.
    Article Snippet: Transcript numbers were expressed as relative to the housekeeping gene b–actin (Applied Biosystems) following the formula 2 DCt ⁄ 105 [9] where DCt = Ctgene of interest Ctb–actin and were corrected for RNA quantity.

    Article Title: Combination drug treatment prolongs survival of experimentally infected mice with silver-haired bat rabies virus.
    Article Snippet: Transcript numbers were expressed relative to the housekeeping gene b–actin (Applied Biosystems) following the formula 2-DCt⁄105 where DCt= Ctgene of interest – Ctb–actin and were corrected for RNA quantity.

    Article Title: Rab from the white shrimp Litopenaeus vannamei: characterization and its regulation upon environmental stress.
    Article Snippet: With the destruction of the ecological environment, shrimp cultivation in China has been seriously affected by outbreaks of infectious diseases.. Rab, which belong to small GTPase Ras superfamily, can regulate multiple steps in eukaryotic vesicle trafficking including vesicle budding, vesicle tethering, and membrane fusion.. Knowledge of Rab in shrimp is essential to understanding regulation and detoxification mechanisms of environmental stress.

    Article Title: Characterization of human nasal organoids from chronic rhinosinusitis patients
    Article Snippet: Absolute transcript copy numbers for each gene and replicate were calculated with the ViiA7 system (Applied Biosystems, CA, USA).

    Article Title: The analysis of lncRNA HOTAIR rs12826786 C>T polymorphism and gastric cancer susceptibility in a Turkish population: lack of any association in a hospital-based case-control study.
    Article Snippet: Background The HOX transcript antisense intergenic RNA (HOTAIR), a well-known long noncoding RNA (lncRNA), has been widely identified to participate in pathogenesis of multiple cancers.. An aberrant up-regulation and biological functions have been observed in gastric cancer (GC).. A common single nucleotide polymorphism (SNP) (rs12826786 C[T) at the HOTAIR has been reported to influence HOTAIR expression, but its association with GC has yet to be investigated in Turkish population.

    TaqMan Allelic Discrimination Assay:

    Article Title: A functional HOTAIR rs12826786 C>T polymorphism is associated with breast cancer susceptibility and poor clinicopathological characteristics in a Turkish population: a hospital-based case-control study.
    Article Snippet: Hox transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is pervasively overexpressed and correlated with tumor invasion, progression, metastasis, and poor prognosis in various human cancers including breast cancer (BC) that plays a role as an oncogenic molecule.. A common functional single-nucleotide polymorphism (SNP) (rs12826786 C>T) at the HOTAIR promoter has been reported to influence HOTAIR expression and gastric adenocarcinoma susceptibility, but relation of HOTAIR rs12826786 C>T polymorphism with BC susceptibility and clinicopathological characteristics has yet to be reported.. To explore the association of theHOTAIR rs12826786 C>T polymorphism with the risk of BC in a Turkish population, a hospital-based case–control study was carried out consisting of 123 BC patients and 122 age-matched healthy controls.

    TaqMan SNP Genotyping Assay:

    Article Title: A functional HOTAIR rs12826786 C>T polymorphism is associated with breast cancer susceptibility and poor clinicopathological characteristics in a Turkish population: a hospital-based case-control study.
    Article Snippet: Hox transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is pervasively overexpressed and correlated with tumor invasion, progression, metastasis, and poor prognosis in various human cancers including breast cancer (BC) that plays a role as an oncogenic molecule.. A common functional single-nucleotide polymorphism (SNP) (rs12826786 C>T) at the HOTAIR promoter has been reported to influence HOTAIR expression and gastric adenocarcinoma susceptibility, but relation of HOTAIR rs12826786 C>T polymorphism with BC susceptibility and clinicopathological characteristics has yet to be reported.. To explore the association of theHOTAIR rs12826786 C>T polymorphism with the risk of BC in a Turkish population, a hospital-based case–control study was carried out consisting of 123 BC patients and 122 age-matched healthy controls.

    Comparison:

    Article Title: Dynamic responses of the benthic bacterial community at the Western English Channel observatory site L4 are driven by deposition of fresh phytodetritus
    Article Snippet: The impact of the seasonal deposition of phytoplankton and phytodetritus on surface sediment bacterial abundance and community composition was investigated at the Western English Channel site L4.. Sediment and water samples were collected from January to September in 2012, increasing in frequency during periods of high water column phytoplankton abundance.. Compared to the past two decades, the spring bloom in 2012 was both unusually long in duration and contained higher than average biomass.

    Software:

    Article Title: Dynamic responses of the benthic bacterial community at the Western English Channel observatory site L4 are driven by deposition of fresh phytodetritus
    Article Snippet: The impact of the seasonal deposition of phytoplankton and phytodetritus on surface sediment bacterial abundance and community composition was investigated at the Western English Channel site L4.. Sediment and water samples were collected from January to September in 2012, increasing in frequency during periods of high water column phytoplankton abundance.. Compared to the past two decades, the spring bloom in 2012 was both unusually long in duration and contained higher than average biomass.

    Reverse Transcription:

    Article Title: Impact of drug distribution into adipose on tissue function: The cholesteryl ester transfer protein (CETP) inhibitor anacetrapib as a test case
    Article Snippet: Tissue mRNA expression in adipose tissue was performed using TaqMan RT‐PCR. mRNA was isolated from adipose tissue using RNeasy mini kit (Qiagen, Germantown) per manufacturer's instructions. .. 2 μg RNA was reverse transcribed using ABI high‐capacity reaction kit and TaqMan PCR was performed using the primer/probe sequences for adiponectin, leptin, serum amyloid A3 (a tissue marker of adipose inflammation), , and beta‐actin as a control (Applied Biosystems/ThermoFisher primer/probe ID numbers: Mm00456425_m1, Mm00440181_m1, Mm00441203_m1, Mm00607939_s1, respectively). ..

    Polymerase Chain Reaction:

    Article Title: Impact of drug distribution into adipose on tissue function: The cholesteryl ester transfer protein (CETP) inhibitor anacetrapib as a test case
    Article Snippet: Tissue mRNA expression in adipose tissue was performed using TaqMan RT‐PCR. mRNA was isolated from adipose tissue using RNeasy mini kit (Qiagen, Germantown) per manufacturer's instructions. .. 2 μg RNA was reverse transcribed using ABI high‐capacity reaction kit and TaqMan PCR was performed using the primer/probe sequences for adiponectin, leptin, serum amyloid A3 (a tissue marker of adipose inflammation), , and beta‐actin as a control (Applied Biosystems/ThermoFisher primer/probe ID numbers: Mm00456425_m1, Mm00440181_m1, Mm00441203_m1, Mm00607939_s1, respectively). ..

    Marker:

    Article Title: Impact of drug distribution into adipose on tissue function: The cholesteryl ester transfer protein (CETP) inhibitor anacetrapib as a test case
    Article Snippet: Tissue mRNA expression in adipose tissue was performed using TaqMan RT‐PCR. mRNA was isolated from adipose tissue using RNeasy mini kit (Qiagen, Germantown) per manufacturer's instructions. .. 2 μg RNA was reverse transcribed using ABI high‐capacity reaction kit and TaqMan PCR was performed using the primer/probe sequences for adiponectin, leptin, serum amyloid A3 (a tissue marker of adipose inflammation), , and beta‐actin as a control (Applied Biosystems/ThermoFisher primer/probe ID numbers: Mm00456425_m1, Mm00440181_m1, Mm00441203_m1, Mm00607939_s1, respectively). ..

    Control:

    Article Title: Impact of drug distribution into adipose on tissue function: The cholesteryl ester transfer protein (CETP) inhibitor anacetrapib as a test case
    Article Snippet: Tissue mRNA expression in adipose tissue was performed using TaqMan RT‐PCR. mRNA was isolated from adipose tissue using RNeasy mini kit (Qiagen, Germantown) per manufacturer's instructions. .. 2 μg RNA was reverse transcribed using ABI high‐capacity reaction kit and TaqMan PCR was performed using the primer/probe sequences for adiponectin, leptin, serum amyloid A3 (a tissue marker of adipose inflammation), , and beta‐actin as a control (Applied Biosystems/ThermoFisher primer/probe ID numbers: Mm00456425_m1, Mm00440181_m1, Mm00441203_m1, Mm00607939_s1, respectively). ..



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    Image Search Results


    Correlations between AMP expression and mucosal small intestinal microbiota revealed a novel microbe capable of inducing Reg3g expression.

    Journal: Gut Microbes

    Article Title: Disentangling the impact of obesity, diet, host factors, and microbiota on small intestinal antimicrobial peptide expression

    doi: 10.1080/19490976.2025.2536095

    Figure Lengend Snippet: Correlations between AMP expression and mucosal small intestinal microbiota revealed a novel microbe capable of inducing Reg3g expression.

    Article Snippet: Different factors can impact AMP expression in the murine small intestine. (a, b, c) Schematic experimental setup of the dietary intervention experiments: (a) Intervention 1, male Charles River mice fed a chow or a high-fat diet (HFD) for 8 weeks; (b) Intervention 2, male and female Charles River mice fed a chow or a Western-style diet (WSD) for 8 weeks; (c) Intervention 3, male and female Taconic mice fed a chow or a WSD for 8 weeks. (d) Total amounts of all measured AMP transcripts in the ileum of all mice fed a chow, WSD, or HFD. (e) Total amounts of all measured AMP transcripts in the ileum of male and female mice fed a chow or WSD from interventions 2 and 3. (f) Reg3g transcript copy number in the ileum of chow and WSD fed mice from interventions 2 and 3. (g) Total amounts of all measured AMP transcripts in the ileum of male Charles River or Taconic mice fed a chow or a WSD. (h) Reg3g transcript copy number in the ileum of male Charles River or Taconic mice fed a chow or a WSD. (i) Total amounts of all measured AMP transcripts in the ileum of female Charles River or Taconic mice fed a chow or a WSD. (j) Reg3g transcript copy number in the ileum of female Charles River or Taconic mice fed a chow or a WSD. (k) Principal component analysis (PCoA) evaluating the effect of diet, mouse vendor, sex, or intervention on AMP expression in mice from the dietary interventions.

    Techniques: Expressing

    Patient demographics and tumor characteristics and association of  ALKBH5  levels with clinicopathological features.

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: Patient demographics and tumor characteristics and association of ALKBH5 levels with clinicopathological features.

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Expressing

    ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence

    Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Transfection, Biomarker Discovery, Over Expression

    Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: CCK-8 Assay, Transwell Assay

    m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Journal: Scientific Reports

    Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

    doi: 10.1038/s41598-024-84352-w

    Figure Lengend Snippet: m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

    Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

    Techniques: Methylation